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human kidney carcinoma rhabdoid tumor cell line  (ATCC)


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    ATCC human kidney carcinoma rhabdoid tumor cell line
    Human Kidney Carcinoma Rhabdoid Tumor Cell Line, supplied by ATCC, used in various techniques. Bioz Stars score: 93/100, based on 34 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/human kidney carcinoma rhabdoid tumor cell line/product/ATCC
    Average 93 stars, based on 34 article reviews
    human kidney carcinoma rhabdoid tumor cell line - by Bioz Stars, 2026-05
    93/100 stars

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    Assessment of morphology, on-target plasmid DNA insertion, and nuclear SMARBC1 expression in knockout clones. (A) Phase contrast images of normal Epi-iPSC, EC6A, and EC7A show no major morphological differences. Scale bar: 25 μm . (B) Target DNA amplification yielded PCR products of 604 bp ( TP53 ) and 341 bp ( SMARCB1 ), implying no on-target plasmid DNA insertion. (C) SMARCB1 staining in two negative control cell <t>lines,</t> <t>CHLA-02-ATRT</t> and CHLA-05-ATRT; one positive control, Epi-iPSC; and two SMARCB1 knockout clones, EC6A and EC7A. Scale bar: 50 μm.
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    Assessment of morphology, on-target plasmid DNA insertion, and nuclear SMARBC1 expression in knockout clones. (A) Phase contrast images of normal Epi-iPSC, EC6A, and EC7A show no major morphological differences. Scale bar: 25 μm . (B) Target DNA amplification yielded PCR products of 604 bp ( TP53 ) and 341 bp ( SMARCB1 ), implying no on-target plasmid DNA insertion. (C) SMARCB1 staining in two negative control cell <t>lines,</t> <t>CHLA-02-ATRT</t> and CHLA-05-ATRT; one positive control, Epi-iPSC; and two SMARCB1 knockout clones, EC6A and EC7A. Scale bar: 50 μm.
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    Assessment of morphology, on-target plasmid DNA insertion, and nuclear SMARBC1 expression in knockout clones. (A) Phase contrast images of normal Epi-iPSC, EC6A, and EC7A show no major morphological differences. Scale bar: 25 μm . (B) Target DNA amplification yielded PCR products of 604 bp ( TP53 ) and 341 bp ( SMARCB1 ), implying no on-target plasmid DNA insertion. (C) SMARCB1 staining in two negative control cell lines, CHLA-02-ATRT and CHLA-05-ATRT; one positive control, Epi-iPSC; and two SMARCB1 knockout clones, EC6A and EC7A. Scale bar: 50 μm.

    Journal: Bioactive Materials

    Article Title: Modeling human brain rhabdoid tumor by inactivating tumor suppressor genes in induced pluripotent stem cells

    doi: 10.1016/j.bioactmat.2023.08.009

    Figure Lengend Snippet: Assessment of morphology, on-target plasmid DNA insertion, and nuclear SMARBC1 expression in knockout clones. (A) Phase contrast images of normal Epi-iPSC, EC6A, and EC7A show no major morphological differences. Scale bar: 25 μm . (B) Target DNA amplification yielded PCR products of 604 bp ( TP53 ) and 341 bp ( SMARCB1 ), implying no on-target plasmid DNA insertion. (C) SMARCB1 staining in two negative control cell lines, CHLA-02-ATRT and CHLA-05-ATRT; one positive control, Epi-iPSC; and two SMARCB1 knockout clones, EC6A and EC7A. Scale bar: 50 μm.

    Article Snippet: The patient-derived ATRT cell lines used were CHLA-02-ATRT (ATCC, Cat#: CRL-3020) from a 20-month-old male and CHLA-05-ATRT (ATCC, Cat#: CRL-3037) from a 2-year-old male.

    Techniques: Plasmid Preparation, Expressing, Knock-Out, Clone Assay, DNA Amplification, Staining, Negative Control, Positive Control

    Immunocytochemistry images for ATRT biomarker detection. ICC was performed for ATRT cell lines CHLA-02-ATRT and CHLA-05-ATRT, normal Epi-iPSC-derived spheroid cells, and EC6A- and EC7A-derived spheroid cells. Separate ICC images for each marker can be found in . NPM: nucleophosmin; GLI1: zinc finger protein GLI1; GLI2: zinc finger protein GLI2; Ki-67: proliferation marker protein Ki-67; Pax-6: paired box protein Pax-6; Oct-4: octamer-binding transcription factor 4; NANOG: homeobox protein NANOG; Hoe: Hoechst 33342. Scale bar: 50 μm.

    Journal: Bioactive Materials

    Article Title: Modeling human brain rhabdoid tumor by inactivating tumor suppressor genes in induced pluripotent stem cells

    doi: 10.1016/j.bioactmat.2023.08.009

    Figure Lengend Snippet: Immunocytochemistry images for ATRT biomarker detection. ICC was performed for ATRT cell lines CHLA-02-ATRT and CHLA-05-ATRT, normal Epi-iPSC-derived spheroid cells, and EC6A- and EC7A-derived spheroid cells. Separate ICC images for each marker can be found in . NPM: nucleophosmin; GLI1: zinc finger protein GLI1; GLI2: zinc finger protein GLI2; Ki-67: proliferation marker protein Ki-67; Pax-6: paired box protein Pax-6; Oct-4: octamer-binding transcription factor 4; NANOG: homeobox protein NANOG; Hoe: Hoechst 33342. Scale bar: 50 μm.

    Article Snippet: The patient-derived ATRT cell lines used were CHLA-02-ATRT (ATCC, Cat#: CRL-3020) from a 20-month-old male and CHLA-05-ATRT (ATCC, Cat#: CRL-3037) from a 2-year-old male.

    Techniques: Immunocytochemistry, Biomarker Discovery, Derivative Assay, Marker, Binding Assay